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pcmv flag n  (TaKaRa)


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    Structured Review

    TaKaRa pcmv flag n
    Pcmv Flag N, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+flag+n/pCMV-DYKDDDDK+Vector+Set/pmc12456019-191-42-44
    Average 94 stars, based on 93 article reviews
    pcmv flag n - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: PRRSV GP5 inhibits the antivirus effects of chaperone-mediated autophagy by targeting LAMP2A
    Article Snippet: .. HSC70, LAMP2A, GFAP and EF1α, which were cloned from the PAM genome (NM_001243907, NM_001244255, XM_005668709, and DQ673096), were ligated into pCMV-Flag-N, pCMV-Myc-N (Clontech, 631604), pEGFP-C3 (Clontech, 6082-1), and/or pmCherry-C1 (Clontech, 632524). ..

    Article Title: PRRSV GP5 inhibits the antivirus effects of chaperone-mediated autophagy by targeting LAMP2A.
    Article Snippet: .. HSC70, LAMP2A, GFAP and EF1α, which were cloned from the PAM genome (NM_001243907, NM_001244255, XM_005668709, and DQ673096), were ligated into pCMV-Flag-N, pCMV-Myc-N (Clontech, 631604), pEGFPC3 (Clontech, 6082-1), and/or pmCherry-C1 (Clontech, 632524). ..

    Article Title: TRIM21 restricts influenza A virus replication by ubiquitination-dependent degradation of M1
    Article Snippet: .. The M1 cDNA fragments from A/Puerto Rico/8/34(PR8), A/swine/Guangdong/04 (H3N2), A/Duck/Fujian/2018 (H5N1), A/Hangzhou/1/2013(H7N9) and A/Chicken/Jiande/09/2009 (H9N2) were cloned separately into vectors pCMV-Myc-N (635689; Clontech, Mountain View, CA, USA), pCMV-Flag-N (635688; Clontech, Mountain View, CA, USA) pCMV-Flag-N-GST, pET-28a-C (69864–3; Novagen, Madison, WI, USA) for different uses. ..

    Article Title: TRIM21 restricts influenza A virus replication by ubiquitination-dependent degradation of M1.
    Article Snippet: .. The M1 cDNA fragments from A/Puerto Rico/8/34(PR8), A/swine/Guangdong/04 (H3N2), A/Duck/Fujian/2018 (H5N1), A/Hangzhou/1/2013(H7N9) and A/Chicken/Jiande/09/2009 (H9N2) were cloned separately into vectors pCMV-Myc-N (635689; Clontech, Mountain View, CA, USA), pCMV-Flag-N (635688; Clontech, Mountain View, CA, USA) pCMV-Flag-N-GST, pET-28aC (69864–3; Novagen, Madison, WI, USA) for different uses. ..

    Transfection:

    Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs.
    Article Snippet: .. Construction of Plasmids and Transfection: DNA fragments encoding full-length gene were amplified from HEK293T cells and individually subcloned into the following expression vectors: pCMV-Myc-N (635 689, Clontech), pCMV-Flag-N (635 689, Clontech), pEGFP-C3 (6082-1, Clontech), mCherry-C1 (632 524, Clontech), pET-28a-C (69864-3, Novagen), and pGEX-4T-1 (27-4580-01, GE Healthcare Biosciences). ..

    Amplification:

    Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs.
    Article Snippet: .. Construction of Plasmids and Transfection: DNA fragments encoding full-length gene were amplified from HEK293T cells and individually subcloned into the following expression vectors: pCMV-Myc-N (635 689, Clontech), pCMV-Flag-N (635 689, Clontech), pEGFP-C3 (6082-1, Clontech), mCherry-C1 (632 524, Clontech), pET-28a-C (69864-3, Novagen), and pGEX-4T-1 (27-4580-01, GE Healthcare Biosciences). ..

    Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs
    Article Snippet: Mouse IFN‐β ELISA Kit (RK00420), Mouse CXCL10/IP10 ELISA Kit (RK00056), Mouse IL‐6 ELISA Kit (RK00008), Mouse TNF‐α ELISA Kit (RK00027), Human IFN‐β ELISA Kit (RK00030), Human CXCL10/IP10 ELISA Kit (RK00054) and Human IL‐6 ELISA Kit (RK00004) were offered by ABclonal. .. DNA fragments encoding full‐length gene were amplified from HEK293T cells and individually subcloned into the following expression vectors: pCMV‐Myc‐N (635 689, Clontech), pCMV‐Flag‐N (635 689, Clontech), pEGFP‐C3 (6082‐1, Clontech), mCherry‐C1 (632 524, Clontech), pET‐28a‐C (69864‐3, Novagen), and pGEX‐4T‐1 (27‐4580‐01, GE Healthcare Biosciences). .. Rabbit polyclonal antibody recognizing phosphorylated API5 pS464 was customized from ABclonal (Wuhan, China).

    Article Title: Heat shock protein A1L restricts influenza A virus by ubiquitination of NA
    Article Snippet: Bafilomycin A1(BafA1; HY-100558), Wortmannin (Wort, HY-10197), MG-132 (HY-13259), and Cycloheximide (CHX, HY-12320) were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. The DNA fragment encoding full-length and truncated variants HSPA1L and NA were separately amplified from HEK293T cells and A/Duck/Fujian/2018 (H5N1), A/Puerto Rico/8/34(PR8), A/swine/Guangdong/ (H3N2), A/Chicken/Jiande/09/2009 (H9N2), and A/Hangzhou/1/2013(H7N9) strain, and were separately subcloned into vectors pCMV-Myc-N (635689; Clontech, Palo Alto, CA, USA), pCMV-Flag-N (635688; Clontech), pET-28a-C (69864-3; Novagen, Madison, WI, USA), pEGFP-C3 (6082-1, Clontech), and pGEX-4T-1 (27-4580-01; GE Healthcare Biosciences, Piscataway, NJ, USA). ..

    Expressing:

    Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs.
    Article Snippet: .. Construction of Plasmids and Transfection: DNA fragments encoding full-length gene were amplified from HEK293T cells and individually subcloned into the following expression vectors: pCMV-Myc-N (635 689, Clontech), pCMV-Flag-N (635 689, Clontech), pEGFP-C3 (6082-1, Clontech), mCherry-C1 (632 524, Clontech), pET-28a-C (69864-3, Novagen), and pGEX-4T-1 (27-4580-01, GE Healthcare Biosciences). ..

    Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs
    Article Snippet: Mouse IFN‐β ELISA Kit (RK00420), Mouse CXCL10/IP10 ELISA Kit (RK00056), Mouse IL‐6 ELISA Kit (RK00008), Mouse TNF‐α ELISA Kit (RK00027), Human IFN‐β ELISA Kit (RK00030), Human CXCL10/IP10 ELISA Kit (RK00054) and Human IL‐6 ELISA Kit (RK00004) were offered by ABclonal. .. DNA fragments encoding full‐length gene were amplified from HEK293T cells and individually subcloned into the following expression vectors: pCMV‐Myc‐N (635 689, Clontech), pCMV‐Flag‐N (635 689, Clontech), pEGFP‐C3 (6082‐1, Clontech), mCherry‐C1 (632 524, Clontech), pET‐28a‐C (69864‐3, Novagen), and pGEX‐4T‐1 (27‐4580‐01, GE Healthcare Biosciences). .. Rabbit polyclonal antibody recognizing phosphorylated API5 pS464 was customized from ABclonal (Wuhan, China).



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